BIOTEC Culture Collection (BCC) was established in 1996 at the National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) to support the national biodiversity policy in conservation and sustainable use of microbial resources in Thailand. The principle practice of the collection is to preserve and collect microbial cultures isolated in Thailand and the accompanying data in a standard system.  

Currently, BCC holds over 18,000 microbial isolates, majority of which is fungal samples isolated from insects, decayed wood, plant seeds, fresh water, soil and leaf litter, lichens, and alkaline sources. Almost all strains in the collection are cryopreserved at –80^oC waiting for screening of bioactive compounds and enzymes. Freeze-drying or storage over liquid nitrogen vapor is used for safe, for strains with special characteristics such as those that produce bioactive compounds, and for safe deposit strains. Duplicate collection of safe deposit strains and strains with special characteristics is held at a different location.

Strain data as well as data regarding storage and supply of cultures are recorded in computers using a program called Microbial Information Management System (MIMS) developed by BIOTEC staff. The program allows rapid searching and retrieving of information relating to the cultures BCC holds.