The paper titled “Simultaneous non-thermal saccharification of cassava pulp by multi-enzyme activity and ethanol fermentation by Candida tropicalis”, authored by Ukrit Rattanachomsri, Sutipa Tanapongpipat, Lily Eurwilaichitr, and Verawat Champreda, was named the JBB Excellent Paper Award for Overseas Researchers 2010. The award was given to the best international paper, selected from papers published in volumes 107 and 108 of the Journal of Bioscience and Bioengineering (JBB) in 2009, which reported outstanding research work conducted by researchers outside Japan.
In this paper, the research team described a novel multi-enzyme process for simultaneous starch release and saccharification under non-thermal conditions as a cost-effective alternative for cassava pulp bioconversion. This offers an alternative to conventional bioprocessing of cassava pulp in which the pulp is subjected to high temperature pre-gelatinization in the presence of an enzyme to release the trapped starch granules, followed by saccharification. However, the conventional saccharification process is energy-intensive and does not release fermentable sugars from the non-starch component of the substrate. The proposed process involves direct hydrolysis and saccharification of cassava pulp using the simultaneous action of multi non-starch polysaccharide hydrolyzing enzymes and raw starch degrading (RSD) amylolytic activity. The optimized conditions were applied in ethanol production using simultaneous saccharification and fermentation (SSF) with the thermotolerant, glucose and xylose-utilizing yeast, Candida tropicalis. This non-thermal saccharification process is promising for application in bioconversion of cassava pulp, a solid by-product from starch processing, to biofuels and other value-added products with improved fermentable sugar yield and energy efficiency.
The paper was published in the Journal of Bioscience and Bioengineering (JBB), vol. 107, issue 5.
Posted on 3 August 2010.