Turbidimeter for detection of shrimp viral infection was developed jointly by Dr. Adisorn Tuantranont, principal researcher of NECTEC and Ms. Wansika Kiatpathomchai, senior researcher of BIOTEC. Wansika and her colleagues at the Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) have acquired expertise in the development of diagnostic probes and kits based on shrimp industry needs to assist in preventing the outbreak of disease. This new platform detects shrimp viruses by using loop-mediated isothermal amplification (LAMP) technique and the result is read by a designed portable multi-channel turbidimeter. In an experiment using Taura Syndrome Virus (TSV) as a model, the detection sensitivity of turbidity measurement was comparable to agarose gel electrophoresis detection of RT-LAMP and nested Real Time-PCR products. Cross reactions with other shrimp viruses were not found, indicating that the RT-LAMP-turbidity measurement was highly specific to TSV and the results could be detected within 1 hour. The new alternative platform is portable and can be used for detection not only in the laboratory but also in the field. This work filed for a Thai patent in March 2011 and was also published in the Journal of Virology Methods in August 2011.
Posted on 20 January 2012