blueAmp: Simple and rapid detection test for streptococcosis in Nile tilapia

Streptococcosis is a major bacterial infection in many cultured fish species, damaging the production of intensive aquaculture species, particularly Nile tilapia. The infection is caused by Streptococcus agalactiae and Strep. Iniae

“blueAmp” has been developed as a simple and rapid assay to detect Strep. agalactiae and Strep. Iniae.  The assay is based on DNA amplification by loop-mediated isothermal amplification (LAMP) and a detection of DNA products by colorimetric change of hydroxynaphthol blue dye. The result can be observed by a color change through the naked eyes.  This method takes approximately an hour, which is significantly less than the time required by a standard test which can take upto 6 days.

“blueAmp” is useful for screening broodstock and fry before stocking and for monitoring fish health in grow-out pond. This technology is developed by Bioengineering and Sensing Technology Laboratory, and is being validated with industry before it becomes available for licensing.

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Posted on 9 April 2015.