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The Bioassay laboratory provides various kinds of screening services to evaluate the biological activities of crude or purified natural products, natural product derivatives and synthetic compounds. The tests can be done at a single or multiple concentrations for the analysis of effective concentration values, e.g. IC50 and MIC. Available services are as follows.

1. Cytotoxicity tests against mammalian cell lines
These assays investigate the ability of compounds to inhibit cell growth. Assays based on fluorometric (resazurin microplate assay) detection of viable cells, or measurement of green fluorescent protein (GFP) expressing cell mass, depending on cell types.

Available cell lines include:

  • KB (human oral cavity carcinoma)
  • MCF-7 (human breast adenocarcinoma)
  • NCI-H187 (human small cell lung, carcinoma)
  • HepG2 (human hepatocarcinoma)
  • Caco-2 (human colon adenocarcinoma)
  • B16-F10 (mouse melanoma)
  • Vero (African green monkey kidney)
  • L929 (mouse subcutaneous connective tissue)
  • BHK21 (baby hamster kidney fibroblast)
  • Human dermal skin fibroblast

2. Anti-human and animal infectious pathogens
The susceptibility of pathogens to compound can be investigated by observing its growth inhibitory effects. The assays provided include anti-malarial parasite (Plasmodium falciparum), anti-TB (Mycobacterium tuberculosis), anti-herpes simplex virus type 1(HSV-1), anti-Candida albicans, anti-Gram positive bacteria (Bacillus cereus and Enterococcus faecium) and anti-Gram negative bacteria (Pseudomonas aeruginosa (PAO1), Acinetobacter baumannii, Klebsiella pneumoniae and Escherichia coli). Furthermore, the new assay against animal pathogen --anti- avian influenza virus assay-- can be determined the ability of compounds to inhibit the activity of Neuraminidase (NA) from H5N1 strain.

3. Anti-plant disease pathogens
These assays investigate the ability of compounds to inhibit fungal spore development based on staining of spore germ-links with a fluorescent dye, carboxy fluorescein diacetate (CFDA). The assays provided are anti-rice blast disease pathogen (Magnaportha grisea), anti-dirty panicle disease pathogen (Curvularia lunata) and anti- leaf spot disease pathogen (Alternaria brassicicola).

4. Mycoplasma testing of cultured cells
The assay tests for the presence of mycoplasma in cell cultures using MycoProbe Mycoplasma Detection kit (R&D systems), which requires a 1.5-ml sample of culture medium from cells grown without antibiotics for at least two weeks

Available services are as follows;

1. anti-Plasmodium falciparuman (K1 strain)
2. anti- Mycobacterium tuberculosis (H37Ra)
3. anti- Bacillus cereus
4. anti- Enterococcus faecium
5. anti- Pseudomonas aeruginosa (PAO1)
6. anti- Acinetobacter baumannii
7. anti- Klebsiella pneumoniae
8. anti- Escherichia coli
9. anti- rice blast disease fungi (Magnaportha grisea)
10. anti-dirty panicle disease fungi (Curvularia lunata)
11. anti- leaf spot disease fungi (Alternaria brassicicola)
12. anti- Candida albicans
13. Neuraminidase (H5N1) inhibition assay
14. anti- Herpes simplex type-1 (HSV-1)
15. Cytotoxicity against to Vero cell (African green monkey kidney fibroblast)
16. Cytotoxicity against to KB cell line (human oral cavity carcinoma)
17. Cytotoxicity against to MCF-7 cell line (human breast adenocarcinoma)
18. Cytotoxicity against to NCI-H187 cell line (human small-cell lung carcinoma)
19. Cytotoxicity against to HepG2 cell line (human hepatocarcinoma)
20. Cytotoxicity against to Caco-2 cell line (human colon adenocarcinoma)
21. Cytotoxicity against to B16-F10 cell line (mouse melanoma)
22. Cytotoxicity against to human dermal skin fibroblast
23. Cytotoxicity against to L929 cell line (mouse subcutaneous connective tissue)
24. Mycoplasma contamination test

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(ลูกค้าสามารถดาวน์โหลดแบบฟอร์มภาษาไทยได้ผ่านทางหน้าเวปไซต์ที่เป็นภาษาไทย)

Customers who wish to submit their samples, please read and accept to our service disclaimer;

Disclaimer
BIOTEC provides preliminary tests for in vitro assessment of biological activities. Test results are limited to our assay conditions and cannot be used for further extrapolation. BIOTEC does not allow the use of test results for commercial advertisements and will not take responsibility for any consequences or damages, which may directly or indirectly result from this information. Please note that BIOTEC is not a certification body. Use of BIOTEC’s name or logo in any case is prohibited.
 

Scientists wish to submit their samples for our assays can mail their samples with the filled request form via postal service to this address:

Bioassay Laboratory
Bioresources Technology Unit
National Center for Genetic Engineering and Biotechnology (BIOTEC)
113 Thailand Science Park, Paholyothin Road
Khlong Nueng, Khlong Luang, Pathum Thani 12120 Thailand

For additional information, please contact: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel: 662 564 6629
Fax: 662 564 6707

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National Center for Genetic Engineering and Biotechnology (BIOTEC)
113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120 THAILAND
Tel: (66-2) 564 6700, Fax : (66-2) 564 6701-5
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