The Bioassay laboratory provides bioactive compound screening services that support research on natural products for BIOTEC laboratories and other research communities in Thailand.  In collaboration with other laboratories in the Bioresources Technology Unit, we developed assays and carry out the screening of natural products from microorganisms.  With an emphasis on fungal derived natural products, our goal is to identify potential lead compounds for further development and utilization in specific areas, including human infectious diseases, crop diseases, and agricultural and domestic animal pests. 

Currently, we have collected over 60,000 extracts derived from over 10,000 microbial isolates in our microbial extract repository.  These extracts are available for future screening of novel biological activities and secondary metabolite profiles.  Through the systematic assessment of microorganisms in BIOTEC Culture Collection, we strive to promote an efficient and sustainable utilization of Thailand natural resources that would ultimately lead to the discovery of natural product entities with potential for commercialization from our microbial resource.

Last Updated (Friday, 24 September 2010 15:32)

 
  1. Investigating the medicinal potentials of microorganisms native to Thailand’s natural habitats
  2. Establishing in vitro bioassays screening for agricultural and animal pest controls
  3. Chemical profile library of non-ribosomal peptides
  4. The eicosanoid biosynthesis pathway and its biological significance

Last Updated (Friday, 24 September 2010 15:16)

 
  1. Cytotoxicity tests against mammalian cell lines
  2. Cytotoxicity tests against insect cell lines
  3. Anti-human and animal infectious pathogens
  4. Anti-plant disease pathogens
  5. Mycoplasma testing of cultured cells

Last Updated (Friday, 29 July 2011 10:20)

 

2012

Supong, K., Thawai, C., Suwanborirux, K., Choowong, W., Supothina, S. and Pittayakhajonwut, P.2012.Antimalarial and antitubercular C-glycosylated benz[α]anthraquinones from the marine-derived Streptomyces sp. BCC45596.Phytochem Lett .5(3):651-656

Bunyapaiboonsri, T.; Vongvilai, P.; Auncharoen, P.; Isaka, M.2012. Cyclohexadepsipeptides from the Filamentous Fungus Acremonium sp. BCC 2629. Helvetica Chimica Acta. 95, 963-972.

Isaka, M.; Srisanoh, U.; Sappan, M.; Supothina, S.; Boonpratuang, T.2012. Sterostreins F-O, illudalanes and norilludalanes from cultures of the Basidiomycete Stereum ostrea BCC 22955. Phytochemistry. 79, 116-120.

Kornsakulkarn, J.; Saepua, S.; Srichomthong, K.; Supothina, S.; Thongpanchang, C.2012. New mycotoxins from the scale insect fungus Aschersonia coffeae Henn. BCC 28712. Tetrahedron. 68, 8480-8486.

2011

Asasutjarit, R., Thanasanchokpibull, S., Fuongfuchat, A. and Veeranondha, S.2011.Optimization and evaluation of thermoresponsive diclofenac sodium ophthalmic in situ gels.INT J PHARMACEUT.411(1-2):128-135

Uppanan, P., Channasanon, S., Veeranondh, S. and Tanodekaew, S.2011.Synthesis of GTMAC modified chitin-PAA gel and evaluation of its biological properties.J Biomed Mater Res A.98A(2):185-191

Boonlarppradab, C., Suriyachadkun, C., Suphothina, S. and Tobwor, P.2011.Bireticulol, a bioactive isocoumarin dimer from Streptomyces sp. BCC24731.J Antibiot.64(3):267-270

Isaka, M., Palasarn, S., Prathumpai, W. and Laksanacharoen, P.2011.Pimarane Diterpenes from the Endophytic Fungus Eutypella sp. BCC 13199.Chem Pharm Bull.59(9):1157-1159

Isaka, M., Yangchum, A., Sappan, M., Suvannakad, R. and Srikitikulchai, P.2011.Cyclohexadepsipeptides from Acremonium sp. BCC 28424.Tetrahedron.67(41):7929–7935

2010

Haritakun, R., Sappan, M., Suvannakad, R., Tasanathai, K. and Isaka, M.2010.An Antimycobacterial Cyclodepsipeptide from the Entomopathogenic Fungus Ophiocordyceps communis BCC 16475.J Nat Prod.73(1):75-78

Bunyapaiboonsri, T., Yoiprommarat, S., Srikitikulchai, P., Srichomthong, K. and Lumyong, S.2010.Oblongolides from the Endophytic Fungus Phomopsis sp. BCC 9789.J Nat Prod.73(1):55-59

Isaka, M., Chinthanom, P., Kongthong, S., Supothina, S. and Ittiworapong, P.2010.Hamigeromycins C–G, 14-membered macrolides from the fungus Hamigera avellanea BCC 17816.tetrahedron.66(4):955-961

2009

Sandee, D., Sivanuntakorn, S., Vichai, V., Kramyu, J., Kirtikara, K.2009.Up-regulation of microsomal prostaglandin E synthase-1 in COX-1 and COX-2 knock-out mouse fibroblast cell lines.PROSTAG OTH LIPID M.88(3-4):111-6

Isaka, M., Srisanoha, U., Veeranondha, S., Choowonga, W. and Lumyong, S.2009.Cytotoxic eremophilane sesquiterpenoids from the saprobic fungus Berkleasmium nigroapicale BCC 8220.TETRAHEDRON.65(43):8808–8815
"

Pittayakhajonwut, P., Usuwan, A., Intaraudom, C., Veeranondha, S. and Srikitikulchai, P.2009.Sesquiterpene Lactone 12,8-Eudesmanolides from the Fungus Xylaria ianthinovelutina.PLANTA MED.75(13):1431-5

Pittayakhajonwut, P., Sri-Indrasutdhi, V., Dramae, A., Lapanun, S., Suvannakad, R. and Tantichareon, M .2009.Graphisins A and B from the Lichen Graphis tetralocularis.AUST J CHEM.62(4):389-91

Isaka, M., Yangchum, A., Rachtawee, P., Khoyaiklang, P., Boonyuen, N. and Lumyong, S.2009.Dihydronaphthalenones from the endophytic fungus Botryosphaeria sp. BCC 8200.Phytochem Lett .2(4):207-210

Bunyapaiboonsri, T., Yoiprommarat, S., Intereya, K., Rachtawee, P., Hywel-Jones, N.L., Isaka, M. .2009.Isariotins E and F, Spirocyclic and Bicyclic Hemiacetals from the Entomopathogenic Fungus Isaria tenuipes BCC 12625.J NAT PROD.72(4):756-9

Chutrakul, C., Boonruangprapa, T., Suvannakad, R., Isaka, M., Sirithunya, P., Toojinda, T. and Kirtikara, K.2009.Ascherxanthone B from Aschersonia luteola, a new antifungal compound active against rice blast pathogen Magnaporthe grisea.J APPL MICROBIOL.107(5):1624-1631

Karoonuthaisiri, N., Charlermroj, R., Uawisetwathana, U., Luxananil, P., Kirtikara, K. and Gajanandana, O.2009.Development of antibody array for simultaneous detection of foodborne pathogens.BIOSENS BIOELECTRON.24(6):1641-8

2008

Sandee D, Sivannuntakorn S, Vichai V, Kramyu J, Kirtikara K (2008) Up-regulation of microsomal prostaglandin E synthase-1 in COX-1 and COX-2 knock-out mouse fibroblast cell lines. Prostaglandins Other Lipid Mediat 88: 111-116.

Berkaew P, Soonthornchareonnon N, Salasawadee K, Chanthaket, R, Isaka M (2008) Aurocitrin and related polyketide metabolites from the wood-decay fungus Hypocrea sp. BCC 14122. J Nat Prod 71: 902–904.

Bunyapaiboonsri T, Veeranondha S, Boonruangprapa T, Somrithipol S (2008) Ramiferin, a bisphenol-sesquiterpene from the fungus Kionochaeta ramifera BCC 7585. Phytochemistry Letters 1:204–206.

Pittayakhajonwut P, Sohsomboon P, Dramae A, Suvannakad R, Lapanun S, Tantichareon M (2008) Antimycobacterial substances from Phaeosphaeria sp BCC8292. Planta medica 74 (3):281-286.

Isaka M, Chinthanom P, Veeranondha S, Supothina S, Luangsa-ard J (2008) Novel cyclopropyl diketones and 14-membered macrolides from the soil fungus Hamigera avellanea BCC 17816. Tetrahedron 64:11028–11033.

2007

Gale GA, Kirtikara K, Pittayakhajonwut P, Sivichai S, Thebtaranonth Y, Thongpanchang C, Vichai V (2007) In search of cyclooxygenase inhibitors, anti-Mycobacterium tuberculosis and anti-malarial drugs from Thai flora and microbes. Pharmacol Ther 115: 307-351.

Bunyapaiboonsri T, Yoiprommarat S, Intereya K, Kocharin K (2007) New diphenyl ethers from the insect pathogenic fungus Cordyceps sp. BCC 1861. Chem Pharm Bull 55(2):304—307.

Isaka M, Boonkhao B, Rachtawee P, Auncharoen P (2007) A xanthocillin-like alkaloid from the insect pathogenic fungus Cordyceps brunnearubra BCC1395. J Nat Prod 70(4):656-658.

2006

Vichai V, Kirtikara K (2006) Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc. 1(3): 1112-1116.

Thongtan J, Saenboonrueng J, Rachtawee P, Isaka M (2006) An antimalarial tetrapeptide from the entomopathogenic fungus Hirsutella sp. BCC 1528. J Nat Prod 69 (4):713–714.

Namdaung U, Aroonrerk N, Suksamrarn S, Danwisetkanjana K, Saenboonrueng J, Arjchomphu W, Suksamrarn A (2006) Bioactive constituents of the root bark of Artocarpus rigidus subsp. rigidus. Chem Pharm Bull (Tokyo) 54(10):1433-1436.

Chinworrungsee M, Kittakoop P, Saenboonrueng J, Kongsaeree P, Thebtaranonth Y (2006) Bioactive compounds from the seed fungus Menisporopsis theobromae BCC 3975. J Nat Prod 69(10):1404-1410.

Rukachaisirikul V, Chantaruk S, Tansakul C, Saithong S, Chaicharernwimonkoon L, Pakawatchai C, Isaka M, Intereya K (2006) A Cyclopeptide from the Insect Pathogenic Fungus Cordyceps sp. BCC 1788. J Nat Prod 69(2):305-307.

2005

Vichai V, Suyarnsesthakorn C, Pittayakhajonwut D, Sriklung K, Kirtikara K (2005) Positive feedback regulation of COX-2 expression by prostaglandin metabolites. Inflamm Res 54:163-72.

Rukachaisirikul V, Tansakul C, Saithong S, Pakawatchai C, Isaka M, Suvannakad R (2005) Hirsutane Sesquiterpenes from the Fungus Lentinus conatus BCC 8996. J Nat Prod 68:1674-1676.

Pittayakhajonwut P, Suvannakad R, Thienhirun S, Prabpai S, Kongsaeree P, Tanticharoen M (2005) An anti-herpes simplex virus-type 1 agent from Xylaria mellisii (BCC 1005). Tetrahed Lett 46(8):1341-1344.

Prajoubklang A, Sirithunyalug B, Charoenchai P, Suvannakad R, Sriubolmas N, Piyamongkol S, Kongsaeree P, Kittakoop P (2005) Bioactive deoxypreussomerins and dimeric naphthoquinones from Diospyros ehretioides fruits: Deoxypreussomerins may not be plant metabolites but may be from fungal epiphytes or endophytes. Chern Biodivers 2(10):1358-1367.

Isaka M, Palasarn S, Kocharin K, Saenboonrueng J (2005) A cytotoxic xanthone dimer from the entomopathogenic fungus Aschersonia sp. BCC 8401. J Nat Prod 68(6):945-6.

Last Updated (Tuesday, 20 November 2012 17:48)