In molecular biology research, microbes are used extensively as production factories for proteins. Microbes are being increasingly employed for industrial manufacture of economically important proteins. Heterologous expression, in which a gene coding for the protein of interest is expressed in an experimentally tractable organism is one of the essential processes to produce target proteins efficiently, which is crucial when large scale production of proteins such as enzymes is required. The technologies for heterologous protein expression have mushroomed in recent years. It has become clear though that there is no single microorganism that can be utilized universally to produce all proteins of interest. The availability of several different host systems would help increase the possibility of success in target protein expression. In our lab, we utilize molecular techniques in order to develop and establish various heterologous expression systems including bacteria, yeast and fungi.