Medical Biotechnology Research Laboratory

The Medical Biotechnology Research Laboratory was established in 1994 as the result of collaborative effort between BIOTEC and the Faculty of Medicine Siriraj Hospital, Mahidol University. The objective of the unit is to maintain expertise in medical biotechnology research, with emphasis on dengue virus and dengue hemorrhagic fever (DHF). The Laboratory consists of two affiliated laboratories located at the Faculty of Medicine Siriraj Hospital and the Faculty of Medicine of Chiang Mai University.

Research Scope

  • Basic research in molecular virology and utilizing the infectious DNA clones of dengue viruses to develop novel dengue vaccine candidates.
  • Basic bio-medical and translational research in the immuno-pathogenesis of DHF, aimed at understanding the complex immunological processes applied to the development of novel predictive diagnostics, technologies for vaccine evaluation and better guidelines of clinical care and public health intervention.

Highlights

  • Discovery of a novel mechanism of T-cell response “original antigenic sin” to dengue virus infection and its correlation with the severity of the disease.
  • Identification of human gene variants associated with dengue fever.
  • Demonstration of the significant functional roles of non-structural protein 1 (NS1) of dengue virus in activation of the complement system, one of the major mechanisms leading to shock and leakage in DHF patients and in evasion of dengue virus from host immune responses. 
  • Demonstration that antibodies against the prM protein caused enhancement of dengue virus infection which should be avoided in dengue vaccine design.
  • Generation of a set of new attenuated dengue vaccine candidates by modification of dengue viral genes using dengue infectious cDNA clones. Some of the vaccine candidates have been licensed to a company for further development. 
  • Establishment of methodology for determining the risk of developing hemorrhagic fever and shock syndrome, by comparing the levels of the dengue virus protein NS1 and the levels, or absence, of the terminal complement complex (SC5b-9).
  • Development of dengue diagnosis: dengue NS1-capture ELISA and a novel Serotyping-NS1 ELISA to identify serotype of dengue virus, based on the utilization of panels of anti-NS1 monoclonal antibodies available in the Unit.
  • Development of a novel reverse and forward genetic systems for large-scale characterization of dengue viruses.
  • Maintaining two long term dengue clinical cohorts aiming at conducting clinical trials as well as translational projects for better care and reduction of mortality.
  • Application of molecular immunology and biology in pathological and immune-electron microscopic investigations in human tissues from autopsy cases.

Major Equipment and Facilities

  • Protein purification facilities: Fast protein liquid chromatography, Low pressure chromatography
  • Cell culture facilities: Laminar flow-biosafety cabinet, CO2 incubator, Fluorescence Microscope
  • Flow cytometry facilities
  • PCR and Real-time PCR
  • KS elispot systems (System for enzyme immuno spot assay evaluations)
  • Laser scanning confocal microscope

Director

Prof. Prida Malasit

Contact Address

Medical Biotechnology Research Laboratory
c/o Medical Molecular Biology Division
Office for Research and Development
12th fl., Adulyadej Vikrom, Siriraj Hospital
Faculty of Medicine
Mahidol University, Prannok
Bangkok 10700 Thailand
Tel: +66 (0) 2419 7000 ext. 6666 to 6670
Fax: +66 (0) 2418 4793
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.