Molecular Biology and Genomics of Shrimp Laboratory was established by Chulalongkorn University in 2003. In 2004, BIOTEC entered into a Memorandum of Understanding with Chulalongkorn University to co-support the activities of the Shrimp Molecular Biology and Genomics Laboratory.
- Genomic research for increasing the culture efficiency of shrimp, including the study of genes and proteins associated with disease resistance.
- Functional genomics approach to identify candidate genes and proteins responding to pathogens in black tiger shrimp Penaeus monodon.
- Molecular mechanisms of immune responses in shrimp.
- Genetic and molecular markers for selective breeding of shrimp at the molecular level (growth, disease resistance, stress).
- Characterization and application of antimicrobial peptides from the black tiger shrimp.
- Study of antiviral immunity of the black tiger shrimp, encompassing characterization of a viral responsive gene and identification of miRNAs involved in white spot syndrome virus (WSSV) infection.
- Molecular mechanisms of melanization/prophenoloxidase system in response to bacterial and viral pathogen infection in shrimp.
- Development of recombinant probiotics for the expression of shrimp antimicrobial peptides and their use in disease control in shrimp aquaculture.
- Development of the Shrimp EST database providing a useful genetic resource to identify tissue-specific genes and gene response to infection and stress, available for download at http://pmonodon.biotec.or.th
- Development of cDNA microarray chip comprised of 9990 different ESTs obtained from the P. monodon EST project was employed to identify viral (white spot and yellow head viruses) and bacterial (Vibrio harveyi) responsive genes in the hemocytes of P. monodon.
- Discovery of the role of anti-lipopolysaccharide factor from the black tiger shrimp in protection from WSSV infection.
- A novel viral responsive protein, Hemocyte Homeostasis-Associated Protein (HHAP), in the black tiger shrimp and its role in the response of shrimp to WSSV infection.
- Identification of the relevant genes involved in shrimp immunity: expression profile of immune-related genes; cathepsins, crustin, penaeidin.
- Characterization of antimicrobial peptides against shrimp pathogens such as penaeidin, crustin, antilipopolysaccharide factors.
- Molecular characteristics of the genes in the proPO system of the penaeid shrimp P. monodon.
- A proposed mechanism for the activation of the proPO cascade in the penaeid shrimp P. monodon.
Major Equipment and Facilities
- Microplate reader with multi-detection system
- FPLC system
- Real time PCR
- Environmentally controlled room for cell and tissue culture
- Fluorescence microscope with digital image gallery and processing
- Digital microscope and image analysis program
Prof. Anchalee Tassanakajon
Department of Biochemistry, Faculty of Science