{"id":2390,"date":"2023-10-27T17:35:14","date_gmt":"2023-10-27T10:35:14","guid":{"rendered":"https:\/\/www.biotec.or.th\/bioassayservice\/?page_id=2390"},"modified":"2025-07-14T10:41:54","modified_gmt":"2025-07-14T03:41:54","slug":"home","status":"publish","type":"page","link":"https:\/\/www.biotec.or.th\/bioassayservice\/en\/home\/","title":{"rendered":"Home"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2390\" class=\"elementor elementor-2390\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-722678b elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"722678b\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-af0f3a2\" data-id=\"af0f3a2\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-03f21e6 elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-pagination-position-inside elementor-widget elementor-widget-slides\" data-id=\"03f21e6\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;both&quot;,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;autoplay_speed&quot;:5000,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;,&quot;transition_speed&quot;:500}\" data-widget_type=\"slides.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper swiper\" role=\"region\" aria-roledescription=\"carousel\" aria-label=\"Slides\" dir=\"ltr\" data-animation=\"fadeInUp\">\n\t\t\t\t<div class=\"swiper-wrapper elementor-slides\">\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-repeater-item-2a971f5 swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"CG in MM_0 min_4\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-13aeaf1 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"13aeaf1\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-9c76569\" data-id=\"9c76569\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-5ea5108\" data-id=\"5ea5108\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a4b4b3b elementor-widget elementor-widget-text-editor\" data-id=\"a4b4b3b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><span style=\"color: #0000ff;\">Bioassay service<\/span><\/h2><p style=\"padding-left: 40px;\">Bioassay research team is part of the National Center for Genetic Engineering and Biotechnology (BIOTEC). We provide bioassay services to evaluate biological activities of compounds against various types of human infectious pathogens and plant pathogens, as well as cytotoxicity assays against mammalian cells.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-94ef010\" data-id=\"94ef010\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-87d217f elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"87d217f\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-abb4897\" data-id=\"abb4897\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-502b2ef\" data-id=\"502b2ef\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-41bca37 elementor-widget elementor-widget-text-editor\" data-id=\"41bca37\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><span style=\"color: #0000ff;\">Routine services<\/span><\/h2><p style=\"padding-left: 40px;\">Different types of services are offered:<\/p><ol><li style=\"list-style-type: none;\"><ol><li style=\"list-style-type: none;\"><ol><li>Positive\/Negative activity test &#8211; performed at a single concentration at 50 \u00b5g\/ml (45-day turnaround time)<\/li><li>Half maximum inhibitory concentration (IC<sub>50<\/sub>) or minimum inhibitory concentration (MIC<sub>90<\/sub>) determination \u2013 performed at 6 concentrations (60-day turnaround time)<\/li><li>IC<sub>50<\/sub> or MIC<sub>90<\/sub> with standard deviation (SD) \u2013 3 independent repeats of assay (90-day turnaround time)<\/li><\/ol><\/li><\/ol><\/li><\/ol><h1 style=\"padding-left: 40px;\">Available assays<\/h1><p style=\"padding-left: 80px;\">1.Cytotoxicity tests against mammalian cell lines<\/p><p style=\"padding-left: 80px;\">Assays are performed using in-house methods based on the evaluation of cell growth inhibition using green fluorescent protein (GFP) detection for Vero cell line and fluorometric (resazurin microplate assay) for other cell lines.\u00a0<\/p><p style=\"padding-left: 40px;\">Available cell lines:<\/p><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul><li>Vero-GFP (African green monkey kidney expressing GFP)<\/li><li>MCF-7 (human breast adenocarcinoma)<\/li><li>NCI-H187 (human small cell lung, carcinoma)<\/li><li>Human dermal fibroblast<\/li><\/ul><\/li><\/ul><\/li><\/ul><ol><li style=\"list-style-type: none;\"><ol><li style=\"list-style-type: none;\"><ol start=\"2\"><li>Anti-human pathogens<\/li><\/ol><\/li><\/ol><\/li><\/ol><p style=\"padding-left: 80px;\">Anti-bacterial and anti-fungal assays are carried out using microplate dilution methods, following the guideline of Clinical and Laboratory Standards Institute (CLSI).\u00a0<\/p><p style=\"padding-left: 40px;\">Available microbial strains:<\/p><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul><li>Bacteria<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 120px;\"><em>Klebsiella pneumoniae<\/em><\/p><p style=\"padding-left: 120px;\"><em>Escherichia coli<\/em><\/p><p style=\"padding-left: 120px;\"><em>Bacillus cereus<\/em><\/p><p style=\"padding-left: 120px;\"><em>Enterococcus faecium<\/em><\/p><p style=\"padding-left: 120px;\"><em>Staphylococcus aureus<\/em><\/p><p style=\"padding-left: 120px;\"><em>Mycobacterium tuberculosis<\/em> (H37Ra)<\/p><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul><li>Fungus<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 120px;\"><em>Candida albicans<\/em><\/p><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul><li>Virus<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 120px;\">Herpes simplex virus type 1 (HSV 1)<\/p><p style=\"padding-left: 120px;\">The anti-HSV 1 test is performed using in-house method. Prior to anti-viral assay, test samples are examined for cytotoxicity using Vero-GFP cell line. The anti-viral activity is determined based on GFP fluorescence detection at the non-cytotoxic concentration range using HSV-1 at 5 MOI.<\/p><ol><li style=\"list-style-type: none;\"><ol><li style=\"list-style-type: none;\"><ol start=\"3\"><li>Anti-plant pathogens<\/li><\/ol><\/li><\/ol><\/li><\/ol><p style=\"padding-left: 120px;\">These assays assess the ability of compounds to suppress fungal spore germination based on staining of spore germlings using an esterase substrate fluorescent dye, carboxy fluorescein diacetate (CFDA).\u00a0<\/p><p style=\"padding-left: 80px;\">Available plant fungal pathogens:<\/p><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul><li><em>Colletotrichum acutatum<\/em><\/li><li><em>Curvularia lunata<\/em><\/li><li><em>Alternaria brassicicola<\/em><\/li><\/ul><\/li><\/ul><\/li><\/ul><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-c6969ba\" data-id=\"c6969ba\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5fb559f elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"5fb559f\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-d93c8c5\" data-id=\"d93c8c5\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-9d5cbe3\" data-id=\"9d5cbe3\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6c70a85 elementor-widget elementor-widget-text-editor\" data-id=\"6c70a85\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><span style=\"color: #0000ff;\">Collaboration opportunities and customized services<\/span><\/h2><p style=\"padding-left: 40px;\">We have over 20 years of experience in natural product screening. Through collaborative projects we have gained expertise on the development of cell-based assays to explore potential applications of bioactive natural products and investigate relevant mechanisms of actions using biochemical assays.<\/p><p style=\"padding-left: 40px;\">We welcome collaborative research opportunities. In addition, we can apply our expertise in developing new assays for specific targets through contract research program.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-306cd18\" data-id=\"306cd18\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6431422 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"6431422\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-032bb38\" data-id=\"032bb38\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-639aac0\" data-id=\"639aac0\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c156fd7 elementor-widget elementor-widget-text-editor\" data-id=\"c156fd7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-size: 18pt; color: #0000ff;\">contact info<\/span><\/p><p style=\"padding-left: 40px;\"><span style=\"font-size: 14pt;\">Phone. 02-5646700 to 3467, 3469 or E-mail : &#x62;&#x69;&#x6f;&#x61;&#x73;&#x73;&#x61;&#x79;&#115;&#101;&#114;&#118;&#105;&#99;&#101;&#64;bio&#x74;&#x65;&#x63;&#x2e;&#x6f;&#x72;&#x2e;&#x74;&#x68;<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-e28309a\" data-id=\"e28309a\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Bioassay service Bioassay research team is part of the National Center for Genetic Engineering and Biotechnology (BIOTEC). We provide bioassay services to evaluate biological activities of compounds against various types of human infectious pathogens and plant pathogens, as well as cytotoxicity assays against mammalian cells. Routine services Different types of services are offered: Positive\/Negative activity &#8230; <a title=\"Home\" class=\"read-more\" href=\"https:\/\/www.biotec.or.th\/bioassayservice\/en\/home\/\" aria-label=\"Read more about Home\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":7,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-2390","page","type-page","status-publish"],"_links":{"self":[{"href":"https:\/\/www.biotec.or.th\/bioassayservice\/wp-json\/wp\/v2\/pages\/2390","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biotec.or.th\/bioassayservice\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.biotec.or.th\/bioassayservice\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.biotec.or.th\/bioassayservice\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biotec.or.th\/bioassayservice\/wp-json\/wp\/v2\/comments?post=2390"}],"version-history":[{"count":30,"href":"https:\/\/www.biotec.or.th\/bioassayservice\/wp-json\/wp\/v2\/pages\/2390\/revisions"}],"predecessor-version":[{"id":2505,"href":"https:\/\/www.biotec.or.th\/bioassayservice\/wp-json\/wp\/v2\/pages\/2390\/revisions\/2505"}],"wp:attachment":[{"href":"https:\/\/www.biotec.or.th\/bioassayservice\/wp-json\/wp\/v2\/media?parent=2390"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}