Microarray technology allows high-throughput screening and analysis. Our laboratory is equipped with technologies to fabricate array chips in-house as well as applying them in various aspects of research and development. Currently, we embark on three main research areas.
Our first area of research employs cDNA microarray technology to study gene expression profiles of various organisms. As Thailand is the world's leading exporter of black tiger shrimp (Penaeus monodon), it is important to understand fundamental biology of this organism. In collaboration with the Molecular Biology and Genetics of Aquatic Animal Laboratory (BIOTEC), we have constructed the first version of cDNA microarray to study the reproduction, nutrigenomics, and stress responses of this economically important animal. Besides using black tiger shrimp as a model organism, we also collaborate with Assoc.Prof. Rasana Wongratanacheewin of Melioidosis Research Center, Faculty of Medicine, Khon Kaen University to employ a whole-genome DNA microarray to investigate genome plasticity of Burkhoderia pseudomallei.
The second area of interest is to develop diagnostic kits by interfacing microarray technology with biological molecules such as antibodies and DNA. In collaboration with the Monoclonal Antibody Laboratory (BIOTEC), we have developed a prototype of antibody array to simultaneously detect multiple foodborne pathogens using chemiluminescent detector. The development of a higher-throughput at a lower cost of this antibody array is underway. Moreover, we aim to develop DNA microarray as a rapid and simple tool to detect bacteria population in various environment for research applications. For instance, we are currently development DNA microarray to detect bacteria community in the shrimp intestine to understand the dynamics of these bacteria and how they affect the well-being of the shrimp.
In the near future, we aim to expand our interests into other technology platforms with high-throughout capacities for research and development. For instance, we have currently employed different metabolomic analysis tools to unravel markers for aroma rice and brown planthopper resistant rice. Once the metabolomic platform is established, it will be further applied for other different organisms for different research proposes.