Samaporn Teeravechyan, Ph.D.
Phone: (66)2-564-6700 ext. 3360, 3361
Having studied HIV entry into host cells during my graduate work, I am interested in the function of the influenza envelope glycoprotein hemagglutinin (HA), its role in viral entry, and its inhibition. My current work focuses on the development of peptides targeting the pre-fusion conformation of HA, development of HA-expressing cell lines, and the use of these cell lines in cell-cell fusion assays to study HA function. In the future, I hope to explore further the interface between virus and its host organisms.
Target-based design and testing of anti-influenza peptides
Antiviral peptides provide a new approach to viral treatment and control. To design peptides capable of inhibiting the entry process of the influenza virus, a genetic algorithm is used to optimize the design of peptides capable of stabilizing the pre-fusion structure of the influenza envelope glycoprotein, hemagglutinin (HA). Peptides generated through this in silico stage would then be tested for inhibitory activity using a general fluorescence-based infectivity assay to detect inhibition of viral replication as well as a cell-cell fusion assay that will allow us to examine directly at the role of the peptides in blocking HA function.
Research Team: Samaporn Teeravechyan, Ekachai Jenwitheesuk, Pavita Thipsombutboon, Sriwan Wongwisansri, Jaraspim Narkpuk
Generation of cell lines stably expressing influenza polymerase complex for antiinfluenza drug screening and protein production in mammalian cells
Increasing resistance to the four available anti-influenza drugs is steadily limiting our options for influenza treatment. To develop a new drug target, we are interested in generating a screening assay for the influenza polymerase complex. Using retroviral transduction, we aim to generate a Vero cell line stably expressing the five necessary components for polymerase activity: the proteins PB2, PB1, PA and NP and negative-sense RNA. The negative-sense RNA will encode a fluorescent reporter, and in the presence of inhibitory compounds, decreased fluorescence will be observed. Furthermore, a cell line expressing only the four proteins will be developed into a tool for high-level protein expression.
Research Team: Samaporn Teeravechyan, Korrawit Jiwsawat, Nanchaya Wanasen, Sriwan Wongwisansri, Jaraspim Narkpuk