Sriwan
Sriwan Wongwisansri, Ph.D.
Researcher
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Phone: (66)2-564-6700 ext. 3474, 3361

I was trained in the field of Molecular Genetics and Biochemistry in the Laybourn’s lab at Colorado State University during 1997-2003. My research works involved using both in vivo and biochemical approaches to study the role of chromatin in PHO5 gene regulation in the level of transcription. Once joined AI lab in 2007, I started to work on developing a mammalian cell line to establish a cell-based system for influenza vaccine production using transfection technology and clonal selection to introduce genes for the avian influenza receptors (ST3GAL1) and the trypsin-like serine protease (TMPRSS2). Currently, we are working on generation of fluorescent-modified influenza as a tool for development of cell-based anti-influenza screening and micro neutralization assays.

My research interests are focused on genetic manipulation to create recombinant viruses, modified mammalian cell lines for many applications, as well as gene expression studies in mammalian cell culture. We mainly utilize co-expression technology (FMDV-2A and ECMV-IRES) for generation of reporter viruses and cells.

Construction and characterization of influenza A virus harboring fluorescent protein as a tool for anti-influenza drug screening and micro neutralization assay

Avian influenza virus was successfully modified to express fluorescent protein in the native NA gene segment. Using reverse genetics and supplement of the exogenous NA from Clostridium perfringens, the recombinant virus NA-DsRed rgH5N1 was generated. After being characterized, the obtained virus was used in the development of cell-based anti influenza screening and micro neutralization assays. Currently, both assays are in the step of optimization and validation.

Research Team: Sriwan Wongwisansri, Jaraspim Narkpuk, Anan Jongkeawwattana

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Jaraspim
Jaraspim Narkpuk, MS
Research assistant
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Phone: (66)2-564-6700 ext. 3467, 3361

Expertise:

Stable mammalian cell line generation, reverse genetics influenza virus production, mammalian cell transfection, flow cytometry, viral plaque assay

Research interests:

Construction and characterization of influenza A virus harboring fluorescent protein as a tool for anti-influenza drug screening and micro neutralization assay

Generation of cell lines stably expressing influenza polymerase complex for anti influenza drug screening and protein production in mammalian cells

Target-based design and testing of anti-influenza peptides

National Center for Genetic Engineering and Biotechnology (BIOTEC)
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Tel: (66-2) 564 6700, Fax : (66-2) 564 6701-5
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