Ray Owens
Protein Production-UK, Research Complex at Harwell, Rutherford Appleton Laboratory and Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
Abstract:
Structural proteomics is the comprehensive analysis of protein structures in the context of whole proteomes. The field originally emerged in response to the acceleration of genomic sequencing and the de-coding of the human genome and that of many pathogens. Over the last ten years, structural proteomics has become a mature discipline focused on specific structural biological problems or the proteomes of specific cells or environments. As one of the first Structural Proteomics Facilities in Europe, we have developed a range of technologies incorporating robotic systems to enable the high throughput cloning, expression and purification of recombinant proteins. Our experience in developing a high throughput pipeline for protein production will be reviewed with reference to recent projects.