A detection system of Listeria monocytogenes in food samples has been developed employing the combination of immunomagnetic separation (IMS) and real-time PCR techniques. L. monocytogenes is isolated from food samples using magnetic beads coated with L. monocytogenes-specific monoclonal antibodies; then the pathogen is detected by real-time PCR technique using specific primers and internal amplification control set. The established system can provide a result within 28 hours compared to 96 hours using the ISO 11290-1:1996 standard method.
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