The ability to determine the sex of cattle embryos before transfer is useful for livestock management, particularly in the dairy cattle industry in which female calves are preferred. The current methods for sexing bovine embryos normally rely on detecting Y chromosome–specific DNA using PCR or fluorescence in situ hybridization (FISH). However, these techniques are not widely used in the field as they are time consuming and labor intensive and require expensive instruments.
BIOTEC research team has developed a modified LAMP in a multiplex format (multiplex LAMP) for highly efficient bovine embryo sexing. The protocol can simultaneously detect both male-specific DNA and control DNA in a single reaction tube using multiplex LAMP. The result can be visualized by the color of the precipitate. The multiplex LAMP showed 100% accuracy in identifying the actual sex of the embryos. The method is cost effective and highly mobile, making it suitable for field use.
This patented assay is available for licensing. For further information, please contact NSTDA Technology Licensing Office (TLO).